Biochemical characteristics of functionally active actin-like protein from liver mitochondria

The actin-like protein with a molecular weight of 42 kDa was obtained from the preparation of freshly isolated mitochondria of the rat liver using the method of immobilized DNAse affinity chromatography. The inhibitory ability of the isolated protein with respect to pancreatic DNAse I was the same a...

Full description

Saved in:
Bibliographic Details
Published inUkrainskij biohimičeskij žurnal Vol. 58; no. 1; p. 5
Main Author Stozharov, A N
Format Journal Article
LanguageRussian
Published Ukraine 01.01.1986
Subjects
Actins - analysis
Actins - pharmacology
Animals
Ca(2+) Mg(2+)-ATPase - metabolism
Chromatography, Affinity
Deoxyribonuclease I - antagonists & inhibitors
Enzyme Activation - drug effects
Female
Kinetics
Male
Mitochondria, Liver - analysis
Molecular Weight
Muscles - analysis
Rabbits
Rats
Online AccessGet more information

Cover

More Information
Summary:The actin-like protein with a molecular weight of 42 kDa was obtained from the preparation of freshly isolated mitochondria of the rat liver using the method of immobilized DNAse affinity chromatography. The inhibitory ability of the isolated protein with respect to pancreatic DNAse I was the same as that of muscular actin. The native structure of the mitochondria protein is confirmed by the data of spectral analysis and its ability to globular-fibrillar transformation with an increased ionic strength of the solution. The polymerization ability as well as a stimulating effect of the actin-like protein of mitochondria on the ATPase activity of myosin is much less pronounced as compared to actin of skeletal muscles.
ISSN:0201-8470