Phenotype and genotype analysis of Torulopsis glabrata isolated from nosocomial infections

Twenty-one strains of Torulopsis glabrata have been isolated in the hospital of Versailles during a three months period, in eight patients, and related to nosocomial infections. In order to know if they have been cross infected, a genotypic analysis (pulsed field electrophoresis) has been performed....

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Bibliographic Details
Published inAnnales de biologie clinique (Paris) Vol. 55; no. 4; p. 323
Main Authors Eloy, O, Lallali, A, Pina, P, Allouch, P Y, Ghnassia, J C
Format Journal Article
LanguageFrench
Published France 01.07.1997
Subjects
Antifungal Agents - pharmacology
Candida - classification
Candida - drug effects
Candida - genetics
Candida - isolation & purification
Cross Infection - microbiology
Electrophoresis, Gel, Pulsed-Field
Genotype
Humans
Multivariate Analysis
Phenotype
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Summary:Twenty-one strains of Torulopsis glabrata have been isolated in the hospital of Versailles during a three months period, in eight patients, and related to nosocomial infections. In order to know if they have been cross infected, a genotypic analysis (pulsed field electrophoresis) has been performed. The strains from four patients had the same karyotype, suggesting they had probably crossed infections. Since this kind of analysis is expensive and time consuming, we have tried a more practical approach. The susceptibility of the strains has been tested by Etest for five antifungal agents: amphotericin B, 5-fluorocytosine, ketoconazole, fluconazole et itraconazole. Statistical analysis of these results has not given the same classification of the strains of T. glabrata. Karyotyping by pulsed field electrophoresis of strains seems to be a better method to confirm strain clonality.
ISSN:0003-3898