A new class of small RNP (alpha-RNP) containing antisense RNA in K-562 cells. IV. The coordinated regulation of the expression of Alu-containing mRNA and alpha-RNA during differentiation

• Published in: Ontogenez Vol. 28; no. 6; p. 437
• Main Authors: Petukhova, O A, Mittenberg, A G, Kulichkova, V A, Kozhukharova, I V, Ermolaeva, Iu B, Gauze, L N, Konstantinova, I M
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.11.1997
• Subjects: Blotting, Northern - methods; Cell Transformation, Neoplastic - drug effects; Cell Transformation, Neoplastic - genetics; Dimethyl Sulfoxide - pharmacology; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - genetics; Human Genome Project; Humans; Leukemia, Erythroblastic, Acute - genetics; Molecular Weight; Nucleic Acid Hybridization - methods; Plasmids - genetics; Repetitive Sequences, Nucleic Acid - drug effects; Repetitive Sequences, Nucleic Acid - genetics; Ribonucleoproteins, Small Nuclear - drug effects; Ribonucleoproteins, Small Nuclear - genetics; RNA, Antisense - drug effects; RNA, Antisense - genetics; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Neoplasm - drug effects; RNA, Neoplasm - genetics; Transcription, Genetic - drug effects; Transcription, Genetic - genetics; Tumor Cells, Cultured
• ISSN: 0475-1450

Small alpha-RNP of K-562 cells contain a small RNA as an RNA component, this RNA is homologous to Alu-repeating sequences of human DNA. When cells are exposed to dimethylsulfoxide, an agent inducing cell differentiation along the erythroid pathway, the content of both high-molecular-weight (heterogeneous nuclear and messenger) RNA enriched with Alu repeats and low-molecular-weight specific RNA, small Alu-homologous alpha-RNA undergoes a coordinated decrease. Using the technique of northern blot hybridization, we have demonstrated nonuniform distribution of Alu repeats both in the fraction of total low-molecular-weight RNA of the cytoplasm as well as in the fraction of messenger RNA. It is proposed that alpha-RNA (alpha-RNP) participates in the control of expression of non-linked Alu-containing genes.