Combination of the sieve effect and ion exchange chromatography during isolation of recombinant alpha2-interferon on the cellosorbent CS KU-23

Preliminary purification of alpha 2-interferon from the culture liquid on cation-exchange sorbents was studied. Application of a cellosorbent based on the cationite KU-23 resulted in a 20-25-fold purification of recombinant alpha 2-interferon in a one-step process as a result of combination of the s...

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Published inPrikladnaja biohimija i mikrobiologija Vol. 33; no. 1; p. 28
Main Authors Demin, A A, Samsonov, G V, Pirogov, V S, Papukova, K P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1997
Subjects
Cellulose
Chromatography, Gel
Chromatography, Ion Exchange
Interferon-alpha - isolation & purification
Recombinant Proteins
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Summary:Preliminary purification of alpha 2-interferon from the culture liquid on cation-exchange sorbents was studied. Application of a cellosorbent based on the cationite KU-23 resulted in a 20-25-fold purification of recombinant alpha 2-interferon in a one-step process as a result of combination of the sieve effect and ion-exchange chromatography.
ISSN:0555-1099