The role of inner membrane in the realization of cAMP-dependent activation of mitochondrial enzymes

It was shown that the increase in the activities of transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase after incubation of mitochondria with cAMP is due to the stimulating effect of cAMP on mitochondria, but not to the increased stability of mitochondria to the incubation procedure. Trea...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 55; no. 2; p. 225
Main Authors Medvedev, A E, Trufanova, L V, Golubenko, A V, Kulinskiĭ, V I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.02.1990
Subjects
Animals
Chloromercuribenzoates - pharmacology
Cyclic AMP - pharmacology
Enzyme Activation
Female
In Vitro Techniques
Intracellular Membranes - enzymology
Isocitrate Dehydrogenase - antagonists & inhibitors
Isocitrate Dehydrogenase - metabolism
Lidocaine - pharmacology
Male
Mitochondria, Liver - enzymology
NADH, NADPH Oxidoreductases - metabolism
NADP Transhydrogenases - antagonists & inhibitors
NADP Transhydrogenases - metabolism
Rats
Rats, Inbred Strains
Trypsin - pharmacology
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Summary:It was shown that the increase in the activities of transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase after incubation of mitochondria with cAMP is due to the stimulating effect of cAMP on mitochondria, but not to the increased stability of mitochondria to the incubation procedure. Treatment of mitochondria with trypsin prevents the action of cAMP on the both enzymes. The integrity of the inner mitochondrial membrane is necessary for the manifestation of cAMP effect. Pretreatment of mitochondria with the local anesthetic, lidocaine, prevents the activation of NAD(P)(+)-transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase during subsequent incubation of mitochondria with cAMP. It is concluded that the role of the inner mitochondrial membrane consists in the reception of the cAMP signal for the internal compartment of mitochondria, i.e. for mitoplasts. Peripheral protein(s) on the external side of the inner mitochondrial membrane seems to play a role in cAMP reception.
ISSN:0320-9725