Flow fluorimetry of the cellular DNA of the bone marrow in normal mice and after exposure to extreme factors

The distribution of murine bone marrow cells in regard to cell cycle was examined using flow cytometry technique. In normal NIH mice the percentage of cells being into phases G1/0, S and G2 + M constitutes 78, 15 and 7%, respectively. In mice subjected to X-irradiation (2, 12 Gy), the thermal burn,...

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Bibliographic Details
Published inT͡S︡itologii͡a Vol. 27; no. 5; p. 541
Main Authors Pereverzev, A E, Rozanov, Iu M, Zenin, V V, Tret'iakov, A N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1985
Subjects
Animals
Bone Marrow - analysis
Bone Marrow - radiation effects
Bone Marrow Cells
Burns - metabolism
Burns - pathology
Cell Division - radiation effects
DNA - analysis
DNA - radiation effects
Flow Cytometry
Interphase - radiation effects
Male
Mice
Mice, Inbred Strains
Time Factors
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Summary:The distribution of murine bone marrow cells in regard to cell cycle was examined using flow cytometry technique. In normal NIH mice the percentage of cells being into phases G1/0, S and G2 + M constitutes 78, 15 and 7%, respectively. In mice subjected to X-irradiation (2, 12 Gy), the thermal burn, and X-irradiation plus the burn the proportion of G2 + M-cells increased, which may be presumably due to their delay on stage G2 of the cell cycle. The start and duration of the delay in the G2 phase depend upon the kind of damage applied.
ISSN:0041-3771