Experiences with the glyoxylic-acid fluorescence method for the demonstration of biogenic amines in cuticle preparations, cryostat- and simple frozen sections

1. Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--...

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Bibliographic Details
Published inZeitschrift fur mikroskopisch-anatomische Forschung Vol. 90; no. 6; p. 1041
Main Authors Stach, W, Jonas, L
Format Journal Article
LanguageGerman
Published Germany 1976
Subjects
Animals
Axons - physiology
Biogenic Amines - metabolism
Cytological Techniques
Freezing
Glyoxylates
Guinea Pigs
Microscopy, Fluorescence - methods
Nervous System - cytology
Rabbits
Rats
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Summary:1. Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--incubation of fresh tissue in glyoxylic acid of 1...2% (pH-value 7.0)--making ready whole mounts, cryostat-or simple frozen sections--air-drying--exposure to 80...100 degrees C for 10...3 minutes--embedding with paraffinum subliquidum. Fluorescence microscopy and photography may be done in the bright and dark field, as well with usual filter-combinations.
ISSN:0044-3107