ColE1 cloning of a ribosomal RNA promoter region from lambdarifd18 by selection for lambda integration and excision functions

The expression of the ribosomal RNA gene carried by the lambda transducing phage lambdarifd18 is shown to be subject to stringent amino acid control. lambdarifd18 DNA was digested with endonuclease EcoRI and ligated to similarly restricted ColE1 plasmid DNA. Selection for expression of lambda integr...

Full description

Saved in:
Bibliographic Details
Published inGene Vol. 2; no. 3-4; p. 159
Main Authors Glaser, G, Enquist, L, Cashel, M
Format Journal Article
LanguageEnglish
Published Netherlands 1977
Subjects
Bacteriocin Plasmids
Coliphages - genetics
DNA, Bacterial - genetics
DNA, Recombinant - genetics
DNA, Recombinant - metabolism
DNA, Viral - genetics
Escherichia coli - genetics
Escherichia coli - metabolism
Guanine Nucleotides - metabolism
Operon
Plasmids
RNA, Ribosomal - biosynthesis
RNA, Ribosomal - genetics
Transduction, Genetic
Online AccessGet full text

Cover

More Information
Summary:The expression of the ribosomal RNA gene carried by the lambda transducing phage lambdarifd18 is shown to be subject to stringent amino acid control. lambdarifd18 DNA was digested with endonuclease EcoRI and ligated to similarly restricted ColE1 plasmid DNA. Selection for expression of lambda integration and excision gene activity carried by the same DNA fragment results in cloning of the promoter proximal portion of the 16S ribosomal RNA gene. The resulting chemera expresses lambda integration and excision functions as well as encoding the promoter proximal half of a 16S ribosomal RNA gene.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-1119
DOI:10.1016/0378-1119(77)90015-4