Isolation of cytochrome b556 from the membranes of Micrococcus lysodeikticus bacteria

• Published in: Biokhimiia (Moscow, Russia) Vol. 41; no. 9; p. 1628
• Main Authors: Lukoianova, M A, Petukhova, N M
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.09.1976
• Subjects: Cytochromes - isolation & purification; Membrane Proteins - isolation & purification; Methods; Micrococcus - analysis

Proteins and cytochrome b556 were solubilized from Micrococcus lysodeikticus membranes using Triton X-100 treatment. Passing of this preparation through DEAE cellulose column in the presence of Triton X-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up...