Isolation of cytochrome b556 from the membranes of Micrococcus lysodeikticus bacteria

Proteins and cytochrome b556 were solubilized from Micrococcus lysodeikticus membranes using Triton X-100 treatment. Passing of this preparation through DEAE cellulose column in the presence of Triton X-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 41; no. 9; p. 1628
Main Authors Lukoianova, M A, Petukhova, N M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1976
Subjects
Cytochromes - isolation & purification
Membrane Proteins - isolation & purification
Methods
Micrococcus - analysis
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Summary:Proteins and cytochrome b556 were solubilized from Micrococcus lysodeikticus membranes using Triton X-100 treatment. Passing of this preparation through DEAE cellulose column in the presence of Triton X-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up to the content of 2.3 nmol per mg of protein. The prostetic group of cytochrome b556 is determined to be protoheme for the spectrum of alkaline pyridinehemochrome.
ISSN:0320-9725