Thermal properties of DNA and polydeoxyribonucleotides in a wide range of ionic concentration of neutral salts and a polymer

deltaHm, deltaT, Tm of melting of polydesoxyribonucletides polydAdT,, polyd(A--T)d(A--T), polyd(A--C)d(T--G) and DNA with different basic composition in a wide range of (10-2--4.0 M) ions (C2H5)4N+, Cs+ and Na+ were determined by the method microcalorimetry. It was established that the difference in...

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Bibliographic Details
Published inBiofizika Vol. 22; no. 5; p. 950
Main Authors Monaselidze, D R, Mgeladze, G N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1977
Subjects
Animals
Cattle
Cesium
Chemical Phenomena
Chemistry
Chlorides
Coliphages
Diethylamines
DNA
DNA, Viral
Hot Temperature
Liver - analysis
Polydeoxyribonucleotides
Rats
Sodium Chloride
Sulfates
Thermodynamics
Thymus Gland - analysis
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Summary:deltaHm, deltaT, Tm of melting of polydesoxyribonucletides polydAdT,, polyd(A--T)d(A--T), polyd(A--C)d(T--G) and DNA with different basic composition in a wide range of (10-2--4.0 M) ions (C2H5)4N+, Cs+ and Na+ were determined by the method microcalorimetry. It was established that the difference in melting heats of deltaHpolydAdT--deltaHpolyd(A--T)d(A--T) being approximately 0.6 kcal/mol b. p. represents that part of the energy which is caused by the heterogeneity of Stacking of interaction between (formula: see text) pairs. It is shown that the narrowing of deltaTm with increasing DNA concentration is connected with the decrease of the difference of polyGC and poly AT melting temperatures, what is with the parameter TGC--TAT which characterizes the melting of a free macromolecule.
ISSN:0006-3029