Selecting a method of erythrocyte fixation for the passive hemagglutination test according to the nature of the sensitin

Quantitative methods were applied to the study of the interaction of albumins, fraction I of Plague bacilli, diphtheria toxoid fractions differing by mol wt, flagellin of typhoid bacilli, 19S- and 7S-fractions of normal human, cholera horse, and paratyphoid B donkey sera with erythrocytes, fixed by...

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Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 2; p. 120
Main Authors Karal'nik, B V, Tsarevskiĭ, Iu P, Shamardin, V A, Shmuter, M F
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.02.1977
Subjects
Adsorption
Animals
Antigens
Erythrocytes - immunology
Hemagglutination Tests - methods
Sheep - blood
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Summary:Quantitative methods were applied to the study of the interaction of albumins, fraction I of Plague bacilli, diphtheria toxoid fractions differing by mol wt, flagellin of typhoid bacilli, 19S- and 7S-fractions of normal human, cholera horse, and paratyphoid B donkey sera with erythrocytes, fixed by 10 different methods. Fixation with acetaldehyde proved to be optimal for the binding of all the proteins, including flagellin, but the latter sensitized erythrocytes formalinized after Vainbach better. The significance of the method of erythrocyte fixation and of the nature of sensitin in the process of the erythrocyte loading without any utilization of the conjugating agents was demonstrated.
ISSN:0372-9311