Properties of a new, oncornavirus D-associated antigen

Continuous cells of human origin, HEp-2, and mouse L929 cells taken 24 hours after infection with oncornavirus D were labeled for protein with a mixture of 14C-amino acids and 3H-glucosamine. Specific sera obtained from rabbits were used for testing the virus-specific new antigen in cell extracts an...

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Bibliographic Details
Published inVoprosy virusologiĭ no. 4; p. 381
Main Authors Kosiakova, N P, Posevaia, T A, Zhdanov, V M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1979
Subjects
Animals
Antigens, Viral - analysis
Antigens, Viral - isolation & purification
Chemical Phenomena
Chemistry, Physical
Electrophoresis, Polyacrylamide Gel - methods
Mice
Molecular Weight
Rabbits
Retroviridae - immunology
Viral Proteins - analysis
Virus Cultivation
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Summary:Continuous cells of human origin, HEp-2, and mouse L929 cells taken 24 hours after infection with oncornavirus D were labeled for protein with a mixture of 14C-amino acids and 3H-glucosamine. Specific sera obtained from rabbits were used for testing the virus-specific new antigen in cell extracts and for immunoprecipitation of the new antigen. Electrophoresis in polyacryl amide gel showed the virusspecific new antigen, common for human HEp-2 cells and for oncornavirus-D-infected mouse L929 cells, to be a glycoprotein with a molecular weight of 98,000--100,000 daltons. By its molecular weight it differs from oncornavirus envelope glycoprotein whose molecular weight is 60,000--70,000 daltons. No similar protein was found in noninfected L929 cells.
ISSN:0507-4088