Allele specificity of genetic recombination in T4 phage using the indicator crossing study method

The diagrams of relative correction ability of eighteen rII mutants of T4 phage were constructed on the basis of two-factor crosses, which were grouped into indicator series. In each series a pair of closely linked compared markers was crossed against indicator ones, the latter being distant enough...

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Bibliographic Details
Published inGenetika Vol. 14; no. 1; p. 111
Main Authors Shcherbakov, V P, Chirkov, G P, Sizova, S T, Efremova, O I, Plugina, L A
Format Journal Article
LanguageRussian
Published Russia (Federation) 1978
Subjects
Alleles
Chromosome Mapping
Coliphages - genetics
Crossing Over, Genetic
Escherichia coli
Methods
Mutation
Recombination, Genetic
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Summary:The diagrams of relative correction ability of eighteen rII mutants of T4 phage were constructed on the basis of two-factor crosses, which were grouped into indicator series. In each series a pair of closely linked compared markers was crossed against indicator ones, the latter being distant enough so as to avoid simultaneous correction with the compared marker. The differences between the frequencies of wild type recombinants in crosses of two compared markers with indicator ones remained constant within the series and can be used as a measure of the differences between the compared markers in their correction ability. Mutants of base substitution type have small but statistically significant differences in correction ability. Simultaneous substitution of two bases in one codon yields a mutant which shows higher correction ability when compared to the mutant obtained as a result of substitution of only one base in the same codon. Frame shift mutants show much wider range of correctibility: some of them are corrected more rarely and others more frequently than base substitution mutants are.
ISSN:0016-6758