Formation of pseudotypes of VSV after culture in a human melanoma

Electron microscope studies showed a high production of melanosomes and viral particles budding into the cisternae of the endoplasmic reticulum in cells derived from a subcutaneous metastasis. The history of this subline (HM6B-A) has been summarized elsewhere. An amelanotic subline (provided by the...

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Bibliographic Details
Published inAnnales de microbiologie Vol. 129; no. 2; p. 217
Main Authors Van Tieghem, N, Vercammen-Grandjean, A, Liteanu, D, Lejeune, F J
Format Journal Article
LanguageFrench
Published France 01.02.1978
Subjects
Antigens, Viral
Cells, Cultured
Humans
Idoxuridine - pharmacology
Inclusion Bodies, Viral
Melanoma - etiology
Melanoma - microbiology
Vesicular stomatitis Indiana virus
Virion
Virus Cultivation
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Summary:Electron microscope studies showed a high production of melanosomes and viral particles budding into the cisternae of the endoplasmic reticulum in cells derived from a subcutaneous metastasis. The history of this subline (HM6B-A) has been summarized elsewhere. An amelanotic subline (provided by the same patient) did not show similar virus particles. When infected with a vesicular stomatitis virus (VSV) thermolabile mutant (tl), these cells produced a VSV pseudotype in which a thermosensitive antigen was modified. Modification of surface VSV antigens was also detected by neutralisation tests. Using these tests, the VSV-pseudotype particles could be used as a tool to detect one or more antigens to a "putative" human melanomavirus, which might be only partially expressed.
ISSN:0300-5410