Electrochemical analysis of microsomal cytochromes

The electrochemical behaviour of the electron transfer proteins -- cytochromes b5, c and P-450 was studied by classical polarography and electrolysis with spectrophotometric monitoring. It was shown electrons are directly transferred from the electrode to oxidized cytochromes c and b5. Cytochrome P-...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 44; no. 9; p. 1569
Main Authors Kuznetsov, B A, Mestechkina, N M, Izotov, M V, Karuzina, I I, Kariakin, A V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1979
Subjects
Animals
Cytochrome c Group - analysis
Cytochrome P-450 Enzyme System - analysis
Cytochromes - analysis
Electrodes
Kinetics
Microsomes, Liver - analysis
Oxidation-Reduction
Polarography
Rabbits
Spectrophotometry
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Summary:The electrochemical behaviour of the electron transfer proteins -- cytochromes b5, c and P-450 was studied by classical polarography and electrolysis with spectrophotometric monitoring. It was shown electrons are directly transferred from the electrode to oxidized cytochromes c and b5. Cytochrome P-450 is not reduced on the electrodes. However, the reduced inactivated from of cytochrome P-420 was detected at high potential values (-1,5 v).
ISSN:0320-9725