Reaction between tetranitromethane and deoxyribonucleoproteins

A comparative kinetic study has been made of tetranitromethane nitration of tyrosine residues in deoxyribonucleoprotein preparation (DNP) treated with EDTA and/or UV light at lambda=253.7 nm, as well as obtained by enzymatic digestion of nucleosomes. UV-light-induced protein-DNA linkages stabilize t...

Full description

Saved in:
Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 43; no. 10; p. 1823
Main Authors Ptitsyn, L A, Chepyzheva, M A, Kolomiĭtseva, G Ia, Senchenkov, E P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.10.1978
Subjects
Chemical Phenomena
Chemistry
Deoxyribonucleoproteins - radiation effects
Edetic Acid
Histones
Kinetics
Methane - analogs & derivatives
Nucleoproteins - radiation effects
Tetranitromethane
Ultraviolet Rays
Online AccessGet more information

Cover

More Information
Summary:A comparative kinetic study has been made of tetranitromethane nitration of tyrosine residues in deoxyribonucleoprotein preparation (DNP) treated with EDTA and/or UV light at lambda=253.7 nm, as well as obtained by enzymatic digestion of nucleosomes. UV-light-induced protein-DNA linkages stabilize the structure of the preparation, whereas the action of chelate agents causes DNP-200 angstrom leads to DNP-100 angstrom transfer. Comparison of kinetic data and the results of the amino acid analysis of individual fractions of nitrated histones allowed to conclude that the differences observed between the degree and the rate of nitration are due to internucleosomic interactions which form the supernucleosomic structure of DNP.
ISSN:0320-9725