Synthesis of human [11-leucineminigastrin I--II. Purification of the synthetic tridecapeptide amide and isolation of a side-product (author's transl)

The preparation of the pure 11-leucine analogue of human minigastrin I, a linear tridecapeptidaemide with full "gastrin activity", from the crude synthetic material obtained after deblocking of the overall protected tridecapeptide amide derivative by means of trifluoroacetic acid, is achie...

Full description

Saved in:
Bibliographic Details
Published inHoppe-Seyler's Zeitschrift für Physiologische Chemie Vol. 359; no. 2; p. 155
Main Authors Jaeger, E, Thamm, P, Schmidt, I, Knof, S, Moroder, L, Wünsch, E
Format Journal Article
LanguageGerman
Published Germany 01.02.1978
Subjects
Amides - isolation & purification
Gastrins - chemical synthesis
Humans
Leucine - analogs & derivatives
Leucine - chemical synthesis
Peptides - isolation & purification
Online AccessGet more information

Cover

More Information
Summary:The preparation of the pure 11-leucine analogue of human minigastrin I, a linear tridecapeptidaemide with full "gastrin activity", from the crude synthetic material obtained after deblocking of the overall protected tridecapeptide amide derivative by means of trifluoroacetic acid, is achieved by ion-exchange chromatography on DEAE-Sephadex A-25 and subsequent partition chromatography on Sephadex G-25. The isolation of a synthetic side-product and its identification as human [10-(Nin-tert-butyl-tryptophan),11-leucine]minigastrin I by means of spectroscopic (UV, fluorescence, 1H-NMR, MS), enzymatic and chromatographic methods is described. The pure tridecapeptide amides human [Leu11]minigastrin I and human [Trp(1'-But)10,Leu11]minigastrin I are isolated in overall yields of 37.4% and 4.6%, respectively (mol-% with regard to the overall protected tridecapeptide amide derivative).
ISSN:0018-4888