Effect of hemolysin from Pseudomonas aeruginosa on cell cultures

Hemolysin of Pseudomonas aeruginosa has a cytopathic action on blood and tissue culture cells. Lysis and disintegration of the architecture of the cell involving membrane and cytoplasm were demonstrated by morphological changes. The hemolytic activity of hemolysin is inhibited by normal sera and by...

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Bibliographic Details
Published inComptes rendus des seances de la Societe de biologie et de ses filiales Vol. 169; no. 1; p. 189
Main Authors Borderon, E, Borderon, J C, Maupas, P
Format Journal Article
LanguageFrench
Published France 1975
Subjects
Blood
Cell Line
Cell Membrane Permeability - drug effects
Cytoplasm - drug effects
gamma-Globulins
Hemolysin Proteins - antagonists & inhibitors
Hemolysin Proteins - metabolism
Hemolysin Proteins - pharmacology
Humans
In Vitro Techniques
Pseudomonas aeruginosa
Serum Albumin
Trypan Blue
Vacuoles - drug effects
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Summary:Hemolysin of Pseudomonas aeruginosa has a cytopathic action on blood and tissue culture cells. Lysis and disintegration of the architecture of the cell involving membrane and cytoplasm were demonstrated by morphological changes. The hemolytic activity of hemolysin is inhibited by normal sera and by albumin; inhibition is also observed in the action of hemolysin on K.B. cells, but this inhibition is not complete. The hemolytic and cytopathic actions are explained by assuming that they alter the molecular architecture of the membranes. The variability may relate to the differing availability of reactive sites on the cells, or indicate that the two activities are associated with two different enzymes.
ISSN:0037-9026