Neutral leucinamidasic activity of human serum. Semiautomatic method of analysis

Half automated method for determining a L-leucinamide splitting enzymatic activity in human sera. In normal and pathological human sera, two distinct L-leucinamide splitting enzymatic activities have been demonstrated. One of them has an optimal activity at pH 9 (alcaline leucine amidase activity) a...

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Bibliographic Details
Published inAnnales de biologie clinique (Paris) Vol. 35; no. 6; p. 459
Main Authors Plaquet, R, Ledeme, N, Vincent-Fiquet, O, Biserte, G
Format Journal Article
LanguageFrench
Published France 1977
Subjects
Autoanalysis - methods
Enzyme Activation
Evaluation Studies as Topic
Humans
Hydrogen-Ion Concentration
Leucyl Aminopeptidase - blood
Magnesium - pharmacology
Reference Values
Temperature
Time Factors
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Summary:Half automated method for determining a L-leucinamide splitting enzymatic activity in human sera. In normal and pathological human sera, two distinct L-leucinamide splitting enzymatic activities have been demonstrated. One of them has an optimal activity at pH 9 (alcaline leucine amidase activity) and shows the most properties of a classic leucinaminopeptidase (E.C. 3.4.11.1). The other (neutral leucine amidase activity) has an optimal activity at pH : 7,5--7,8 and is not activated by Mg2+ ions. In the present work a semi-automated method permitting the determination of the "neutral leucine amidase activity" is presented. The mean of the reference values for normal human sera are established to 31,54 mUI/ml, and the upper normal limit is 48 mUI/ml. The neutral leucine amidase activity is studied in pathological sera comparatively with two other aminopeptidase activities : "alcaline leucine amidase activity", and "leucine-arylamidase". Our study shows that in pathological sera, the neutral leucine amidase activity" varies often without any correlation with those parameters.
ISSN:0003-3898
1950-6112