Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities

We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1319; p. 245
Main Authors Zhang, Keya, Bhuripanyo, Karan, Wang, Yiyang, Yin, Jun
Format Journal Article
LanguageEnglish
Published United States 2015
Subjects
Catalysis
Cell Separation
Cell Surface Display Techniques
Enzymes - chemistry
Enzymes - genetics
Enzymes - metabolism
Flow Cytometry
Peptide Library
Protein Binding
Protein Engineering - methods
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Substrate Specificity
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Summary:We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and fluorescence-activated cell sorting.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2748-7_14