Identification of 13,14‐dihydro‐15‐oxo‐prostaglandin F2α in the circulation during infusions of bradykinin and prostaglandin E2 in man

• Published in: British journal of pharmacology Vol. 91; no. 1; pp. 245 - 250
• Main Authors: Barrow, S.E., Cockcroft, J., Dollery, C.T., Hickling, N.E., Ritter, J.M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.1987; Nature Publishing
• Subjects: Adult; Biological and medical sciences; Biotransformation; Bradykinin - metabolism; Dinoprost - analogs & derivatives; Dinoprostone; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Humans; Male; Prostaglandins E - metabolism; Prostaglandins F - blood; Prostaglandins. Arachidonic acid metabolites; Vertebrates: endocrinology; Human; Prostaglandin E2; Identification; Analog; Arachidonic acid derivatives; Bradykinin
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1987.tb09005.x

1 Bradykinin, prostaglandin E2 (PGE2), PGD2 and vehicle (saline) were each administered intravenously on separate occasions to 6 healthy men for a period of 60 min. 2 13,14‐Dihydro‐15‐oxo‐PGF2α was identified in plasma samples obtained during intravenous infusions of bradykinin and PGE2 but not during infusions of PGD2 or saline. 3 The structure of this metabolite was verified by comparison of three different derivatives with authentic standards, using gas chromatography/electron capture mass spectrometry. 4 Bradykinin increased plasma concentrations of 13,14‐dihydro‐15‐oxo‐PGF2α from baseline values in the range < 5–10 pg ml−1 to 28–403 pg ml−1. PGE2 increased plasma concentrations of 13,14‐dihydro‐15‐oxo‐PGF2α from baseline values in the range < 5–17 pg ml−1 to 160–603 pg ml−1. Neither PGD2 nor the vehicle affected 13,14‐dihydro‐15‐oxo‐PGF2α concentrations. 5 We conclude that bradykinin‐stimulated 13,14‐dihydro‐15‐oxo‐PGF2α may be derived from PGE2 or PGF2α. The possibility that these prostaglandins are synthesized by stimulation of microvascular endothelium during bradykinin infusion is discussed.