A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 int...

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Published inBMC biotechnology Vol. 14; no. 1; p. 51
Main Authors Fayed, Bahgat, Younger, Ellen, Taylor, Gabrielle, Smith, Margaret C M
Format Journal Article
LanguageEnglish
Published England BioMed Central 30.05.2014
Subjects
Attachment Sites, Microbiological - genetics
Bacteria
Bacteriology
Bacteriophages - genetics
Base Sequence
Biotechnology
Bulk molding compounds
Cloning
Deoxyribonucleic acid
DNA
E coli
Enzymes
Experiments
Genes
Genetic engineering
Genetic Vectors - metabolism
Genetics
Genomes
Integrases - chemistry
Integrases - genetics
Integrases - metabolism
Mathematical analysis
Medical research
Molecular Sequence Data
Phage
Plasmids
Polymerase chain reaction
Sequence Alignment
Streptomyces
Streptomyces - metabolism
Vectors (mathematics)
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Summary:Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1472-6750
1472-6750
DOI:10.1186/1472-6750-14-51