FcRγ‐chain deficiency reduces the development of diet‐induced obesity
Objective Pathogenic immunoglobulins are produced during the development of obesity and contribute to the development of insulin resistance (IR). However, the mechanisms by which these antibodies affect IR are largely unknown. This study investigated whether Fc‐receptors contribute to the developmen...
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Published in | Obesity (Silver Spring, Md.) Vol. 23; no. 12; pp. 2435 - 2444 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.12.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Objective
Pathogenic immunoglobulins are produced during the development of obesity and contribute to the development of insulin resistance (IR). However, the mechanisms by which these antibodies affect IR are largely unknown. This study investigated whether Fc‐receptors contribute to the development of diet‐induced obesity and IR by studying FcRγ−/− mice that lack the γ‐subunit necessary for signaling and cell surface expression of FcγR and FcεRI.
Methods
FcRγ−/− and wild‐type (WT) mice were fed a high‐fat diet (HFD) to induce obesity. At 4 and 11 weeks, body weight and insulin sensitivity were measured, and adipose tissue (AT) inflammation was determined. Furthermore, intestinal triglyceride (TG) uptake and plasma TG clearance were determined, and gut microbiota composition was analyzed.
Results
FcRγ−/− mice gained less weight after 11 weeks of HFD. They had reduced adiposity, adipose tissue inflammation, and IR. Interestingly, FcRγ−/− mice had higher lean mass compared to WT mice, which was associated with increased energy expenditure. Intestinal TG absorption was increased whereas plasma TG clearance was not affected in FcRγ−/− mice. Gut microbial composition differed significantly and might therefore have added to the observed phenotype.
Conclusions
FcRγ‐chain deficiency reduces the development of diet‐induced obesity, as well as associated AT inflammation and IR at 11 weeks of HFD. |
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Bibliography: | Disclosure Funding agencies The authors declare no conflict of interest. This work was supported by grants from the Center of Medical Systems Biology (CMSB), the Leiden University Medical Center, and Rembrandt Institute of Cardiovascular Science (RICS). PCNR is Established Investigator of the Netherlands Heart Foundation (grant 2009T038). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1930-7381 1930-739X |
DOI: | 10.1002/oby.21309 |