Controlled confinement of DNA at the nanoscale: nanofabrication and surface bio-functionalization

Nanopatterned arrays of biomolecules are a powerful tool to address fundamental issues in many areas of biology. DNA nanoarrays, in particular, are of interest in the study of DNA-protein interactions and for biodiagnostic investigations. In this context, achieving a highly specific nanoscale assemb...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 749; p. 169
Main Authors Palma, Matteo, Abramson, Justin J, Gorodetsky, Alon A, Nuckolls, Colin, Sheetz, Michael P, Wind, Shalom J, Hone, James
Format Journal Article
LanguageEnglish
Published United States 01.01.2011
Subjects
DNA - chemistry
DNA - metabolism
DNA-Binding Proteins - metabolism
Glass - chemistry
Gold - chemistry
Immobilization - methods
Microscopy, Atomic Force
Nanostructures - chemistry
Nanotechnology - methods
Palladium - chemistry
Surface Properties
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Summary:Nanopatterned arrays of biomolecules are a powerful tool to address fundamental issues in many areas of biology. DNA nanoarrays, in particular, are of interest in the study of DNA-protein interactions and for biodiagnostic investigations. In this context, achieving a highly specific nanoscale assembly of oligonucleotides at surfaces is critical. In this chapter, we describe a method to control the immobilization of DNA on nanopatterned surfaces; the nanofabrication and the bio-functionalization involved in the process will be discussed.
ISSN:1940-6029
DOI:10.1007/978-1-61779-142-0_12