Direct sequencing from low-melt agarose with Sequenase

Recently a method was described in which PCR products are sequenced directly using Taq polymerase. Here, the authors describe a method in which PCR products purified in low-melt agarose can be sequenced directly using Sequenase. This allows the PCR product to be prepared for direct sequencing in a s...

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Bibliographic Details
Published inNucleic acids research Vol. 17; no. 14; p. 5864
Main Authors KRETZ, K. A, CARSON, G. S, O'BRIEN, J. S
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.07.1989
Subjects
agarose
Base Sequence
Biological and medical sciences
Biotechnology
DNA - genetics
DNA-Directed DNA Polymerase
Fundamental and applied biological sciences. Psychology
Gene Amplification
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
phage T7
polymerase chain reaction
Sepharose
Synthetic digonucleotides and genes. Sequencing
Polymerase chain reaction
Nucleotide sequence
Enzyme
Process improvement
DNA
Genetic engineering
Method
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Summary:Recently a method was described in which PCR products are sequenced directly using Taq polymerase. Here, the authors describe a method in which PCR products purified in low-melt agarose can be sequenced directly using Sequenase. This allows the PCR product to be prepared for direct sequencing in a single step.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.14.5864