Preimplantation genetic diagnosis of aneuploidy: were we looking at the wrong chromosomes?

• Published in: Journal of assisted reproduction and genetics Vol. 16; no. 4; pp. 176 - 181
• Main Authors: Bahçe, M, Cohen, J, Munné, S
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.04.1999; Kluwer Academic Publishers-Plenum Publishers
• Subjects: Adult; Age groups; Aneuploidy; Chromosomes; Chromosomes, Human; Embryo, Mammalian - physiology; Embryos; Female; Humans; Hybridization; In Situ Hybridization, Fluorescence; Maternal Age; Miscarriage; Monosomy; Morphology; Pregnancy; Preimplantation Diagnosis; Trisomy
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1023/A:1020308722247

Our purpose was to study aneuploidy frequencies of chromosomes 1, 4, 6, 7, 14, 15, 17, 18, and 22 in cleavage-stage embryos. These frequencies were compared to spontaneous abortion data to determine differences in survival rate of their aneuploidies. One hundred ninety-four embryos were analyzed with multicolor fluorescence in situ hybridization. Embryos were divided into three maternal age groups: 20 to 34.9 years, (2) 35 to 39.9 years, and (3) 40 years and older. Embryos were also divided into two developmental and morphological groups; arrested and nonarrested embryos. The rate of aneuploidy was 14.51%, 14.10%, and 31.48% for age groups 1, 2, and 3, respectively (P < 0.005). The chromosomes most frequently involved in aneuploidy events were 22, 15, 1, and 17. The chromosomes most involved in spontaneous abortions are not necessarily the ones causing a decrease in implantation rates with maternal age. Other aneuploidies, such as for chromosomes 1 and 17, may seldom implant or die shortly after implantation.