Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae

Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4 Escherichia coli (F4 ETEC) and isolated F4 fimbriae. The aim of this stud...

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Published inBMC genetics Vol. 15; no. 1; p. 103
Main Authors Goetstouwers, Tiphanie, Van Poucke, Mario, Coddens, Annelies, Nguyen, Van Ut, Melkebeek, Vesna, Deforce, Dieter, Cox, Eric, Peelman, Luc J
Format Journal Article
LanguageEnglish
Published England BioMed Central 03.10.2014
Subjects
Amino acids
Animals
Bacterial Adhesion
Binding sites
Carbohydrates
Chromosomes
E coli
Escherichia coli
Escherichia coli - physiology
Fimbriae, Bacterial - metabolism
Genes
Genotype & phenotype
Glycosphingolipids - biosynthesis
Glycosylation
Glycosyltransferases - genetics
Laboratories
Microvilli - microbiology
Mutation
Studies
Sus scrofa - genetics
Veterinary medicine
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Summary:Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4 Escherichia coli (F4 ETEC) and isolated F4 fimbriae. The aim of this study was to investigate the hypothesis that variation in genes involved in the assembly of the F4 binding carbohydrate moiety of GSLs (i.e. ARSA, B4GALT6, GAL3ST1, GALC, GBA, GLA, GLB1, GLB1L, NEU1, NEU2, UGCG, UGT8) could account for differential binding of F4 ETEC and their fimbriae. RT-PCR could not reveal any differential expression of the 12 genes in the jejunum of F4 receptor-positive (F4R(+)) and F4 receptor-negative (F4R(-)) pigs. Sequencing the complete open reading frame of the 11 expressed genes (NEU2 was not expressed) identified 72 mutations. Although some of them might have a structural effect, none of them could be associated with a F4R phenotype. We conclude that no regulatory or structural variation in any of the investigated genes is responsible for the genetic susceptibility of pigs towards F4 ETEC.
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ISSN:1471-2156
1471-2156
DOI:10.1186/s12863-014-0103-x