Targeted disruption of sti35, a stress-responsive gene in phytopathogenic fungus Fusarium oxysporum

• Published in: Current microbiology Vol. 41; no. 4; pp. 284 - 289
• Main Authors: THANONKEO, Pornthap, AKIYAMA, Kouichi, JAIN, Sona, TAKATA, Renkichi
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.10.2000; Springer Nature B.V
• Subjects: Biological and medical sciences; Carbohydrates; Culture Media; Development; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fungi; Fusarium - chemistry; Fusarium - genetics; Fusarium - growth & development; Fusarium oxysporum; Genes, Fungal; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Heat treatment; Heat-Shock Proteins - genetics; Hot Temperature; Microbiology; Mycology; Phytopathology. Animal pests. Plant and forest protection; Plant diseases; Plants - microbiology; Point Mutation; sti35 gene; Temperature; Variation, races, biotypes, parasitic specialization, genetics; Fungi; Fusarium oxysporum; Plant pathogen; Heat shock protein; Vegetal microorganism relation; Property structure relationship; Fungi Imperfecti; Mutation; Thermotolerance; Thallophyta
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s002840010135

sti35 is one of the heat-shock genes in Fusarium oxysporum, which is a fungal pathogen for wilt disease in plants. We have isolated a genomic clone of sti35 and used it to create disruption mutations. Disruption of the sti35 coding region resulted in the loss of a 32-kDa protein present in heat-shocked cells. The disruption had no detectable effect on growth and development at various temperatures, nor on the ability to acquire thermotolerance in nutrient medium. But the sti35 disruptants showed increased thermotolerance, relative to the wild-type strain, when incubated in minimal medium after heat treatment.