Suppression of humoral immune response to hepatitis B surface antigen vaccine in BALB/c mice by 1-methyl-tryptophan co-administration

• Published in: Daru Vol. 19; no. 3; pp. 236 - 239
• Main Authors: Eleftheriadis, T, Sparopoulou, T, Antoniadi, G, Liakopoulos, V, Stefanidis, I, Galaktidou, G
• Format: Journal Article
• Language: English
• Published: Switzerland Springer Nature B.V 2011; Tehran University of Medical Sciences
• Subjects: Original; DL-1-MT; IDO; Serum anti-HBs
• ISSN: 1560-8115; 2008-2231

Indoleamine 2,3-dioxygenase (IDO) suppresses adaptive immune response. The purpose of this study was to determine the effect of the IDO inhibitor namely 1-methyl-DL-tryptophan (DL-1-MT) on antibody production after vaccination with hepatitis B surface (HBs) antigen. Four groups of BALB/c mice were immunized with a HBs antigen vaccine. In the first group the vaccine had no DL-1-MT, whereas in the other three groups the vaccine contained 1 mg, 10 mg and 20 mg DL-1-MT. Blood samples were collected 5 weeks post-vaccination and anti-HBs antibodies in the serum were measured by ELISA. Compared to the three groups of mice that were immunized with the vaccines containing DL-1-MT, serum anti-HBs level was much higher in the mice that were immunized with the vaccine with out DL-1-MT. Inhibition of IDO at the time of vaccination decreased humoral immune response to HBs antigen vaccine. The idea that IDO activity is simply immunosuppressive may need to be re-evaluated.