Characterization of IS18, an element capable of activating the silent aac(6')-Ij gene of Acinetobacter sp. 13 strain BM2716 by transposition

• Published in: Antimicrobial agents and chemotherapy Vol. 42; no. 10; pp. 2759 - 2761
• Main Authors: RUDANT, E, COURVALIN, P, LAMBERT, T
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1998
• Series: Note
• Subjects: Acinetobacter; Acinetobacter - genetics; Acinetobacter baumannii; Acinetobacter haemolyticus; Amino Acid Sequence; Aminoglycosides; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteriology; Base Sequence; Biological and medical sciences; DNA Transposable Elements; Drug Resistance, Microbial - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Mechanisms of Resistance; Medical sciences; Microbiology; Molecular Sequence Data; Pharmacology. Drug treatments; Resistance; Transposition; Nucleotide sequence; Aminoglycoside; Neisseriaceae; Silent allele; Bacteria; Micrococcales; Acinetobacter; Gene expression; In vitro
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/aac.42.10.2759

Insertion sequence IS18 was detected by analysis of the spontaneous aminoglycoside resistant mutant Acinetobacter sp. 13 strain BM2716-1. Insertion of the element upstream from the silent acetyltransferase gene aac(6')-Ij created a hybrid promoter that putatively accounts for the expression of the aminoglycoside resistance gene. The 1, 074-bp IS18 element contained partially matched (20 out of 26 bases) terminal inverted repeats, one of which overlapped the 3' end of a 935-bp open reading frame potentially encoding a protein related to the transposases of the IS30 family. IS18 was found in 6 out of 29 strains of Acinetobacter sp. 13 but not in 10 strains each of A. baumannii and A. haemolyticus.