Studies of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Effect of inhibitors and low temperature

• Published in: Yeast (Chichester, England) Vol. 14; no. 11; pp. 1007 - 1016
• Main Authors: Ducrey Sanpietro, Luis M., Kula, M.‐R.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.1998
• Subjects: Antioxidants; astaxanthin biosynthesis; Basidiomycota - drug effects; Basidiomycota - growth & development; Basidiomycota - metabolism; beta Carotene - analogs & derivatives; beta Carotene - biosynthesis; beta Carotene - metabolism; Carotenoids - metabolism; Cycloheximide - pharmacology; diphenylamine; Diphenylamine - pharmacology; Dose-Response Relationship, Drug; nicotine; Nicotine - pharmacology; Pfaffia rhodozyma; Temperature; Time Factors; Xanthophyllomyces dendrorhous; Xanthophylls
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/(SICI)1097-0061(199808)14:11<1007::AID-YEA307>3.0.CO;2-U

The effect of nicotine and diphenylamine on astaxanthin biosynthesis in Xanthophyllomyces dendrorhous was studied. The effects were analysed under standard and low temperature conditions. It was found that 10 mm‐nicotine inhibits the cyclization of lycopene and de novo protein synthesis was not needed to reverse the inhibition. The oxidation of β‐carotene was irreversibly inhibited by 10 μM‐diphenylamine while the dehydrogenation of phytoene was reversibly inhibited by 60 μM‐diphenylamine. The simultaneous exposure to low temperature (4°C) overcomes the inhibition of β‐carotene oxidation at low diphenylamine concentration. © 1998 John Wiley & Sons, Ltd.