Prezygotic mate selection is only partially correlated with the expression of NaS‐like RNases and affects offspring phenotypes

• Published in: The New phytologist Vol. 242; no. 6; pp. 2832 - 2844
• Main Authors: Baraniecka, Patrycja, Seibt, Wibke, Groten, Karin, Kessler, Danny, McGale, Erica, Gase, Klaus, Baldwin, Ian T., Pannell, John R.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.06.2024
• Subjects: Ethene; Ethylene; Genomes; Genotypes; Inbreeding; Mate selection; Nicotiana attenuata; Offspring; Phenotypes; Pollen; Pollination; postpollination ethylene burst; Progeny; Recombinants; Reproductive behaviour; reproductive strategy; self‐incompatibility; Sexual selection; S‐like‐RNase; trade‐off; Nicotiana attenuata; reproductive strategy; mate selection; postpollination ethylene burst; S‐like‐RNase; trade‐off; self‐incompatibility
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.19741

Summary Nicotiana attenuata styles preferentially select pollen from among accessions with corresponding expression patterns of NaS‐like‐RNases (SLRs), and the postpollination ethylene burst (PPEB) is an accurate predictor of seed siring success. However, the ecological consequences of mate selection, its effect on the progeny, and the role of SLRs in the control of ethylene signaling remain unknown. We explored the link between the magnitude of the ethylene burst and expression of the SLRs in a set of recombinant inbred lines (RILs), dissected the genetic underpinnings of mate selection through genome‐wide association study (GWAS), and examined its outcome for phenotypes in the next generation. We found that high levels of PPEB are associated with the absence of SLR2 in most of the tested RILs. We identified candidate genes potentially involved in the control of mate selection and showed that pollination of maternal genotypes with their favored pollen donors produces offspring with longer roots. When the maternal genotypes are only able to select against nonfavored pollen donors, the selection for such positive traits is abolished. We conclude that plants' ability of mate choice contributes to measurable changes in progeny phenotypes and is thus likely a target of selection.