Mass spectrometric analysis of leukotriene A4 and other chemically reactive metabolites of arachidonic acid

• Published in: Journal of the American Society for Mass Spectrometry Vol. 13; no. 10; pp. 1227 - 1234
• Main Authors: DICKINSON, Jennifer S, MURPHY, Robert C
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 01.10.2002; Springer Nature B.V
• Subjects: 6-Ketoprostaglandin F1 alpha - analysis; Activation; Analytical, structural and metabolic biochemistry; Anions; Arachidonic acid; Arachidonic Acid - chemistry; Arachidonic Acid - metabolism; Biological and medical sciences; Biosynthesis; Chemical attack; Chemistry; Chromatography, High Pressure Liquid; Columns (structural); Eicosanoids - analysis; Epoprostenol - analysis; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Half-life; Hydrogen-Ion Concentration; Indicators and Reagents; Ionization; Ions; Leukotriene A4 - analysis; Mass spectrometry; Metabolites; Online Systems; Organic chemistry; Other biological molecules; Oxygen Isotopes; Plant growth substances; Prostaglandin H2; Prostaglandins; Prostaglandins H - analysis; Quadrupoles; Reactivity and mechanisms; Spectrometry; Spectrometry, Mass, Electrospray Ionization; Elution; Gradient; Collisional activation; Chemical analysis; Prostaglandin I2; Metabolite; Arachidonic acid derivatives; Prostaglandin H2; HPLC chromatography; Prostaglandin E2; Fragmentation pattern; Prostaglandin D2; Electrospray; Leukotriene; Reversed phase chromatography; Analysis method; Eicosanoid; Mass spectrometry MS/MS; Mass spectrometry; Structural analysis

The biosynthesis of prostaglandins and leukotrienes proceeds through the formation of chemically reactive intermediates leukotriene A4 (LTA4) and prostaglandin H2 (PGH2) which in aqueous solutions have chemical half-lives of 3 s and 3 min, respectively. Prostacyclin (PGI2) is another chemically reac...