Motility of Campylobacter concisus isolated from saliva, feces, and gut mucosal biopsies

• Published in: APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 125; no. 3; pp. 230 - 235
• Main Authors: Ovesen, Sandra, Kirk, Karina Frahm, Nielsen, Hans Linde, Nielsen, Henrik
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01.03.2017
• Subjects: Biopsy; Campylobacter - pathogenicity; Campylobacter concisus; Campylobacter fetus; Campylobacter Infections - microbiology; Campylobacter jejuni; Fetuses; flagella; gastroenteritis; Gastroenteritis - microbiology; Humans; inflammatory bowel disease; Inflammatory Bowel Diseases - microbiology; Motility; Poultry; Virulence; flagella; Motility; Campylobacter concisus; gastroenteritis; inflammatory bowel disease
• ISSN: 0903-4641; 1600-0463
• DOI: 10.1111/apm.12655

Campylobacter concisus is an emerging pathogen associated with gastrointestinal disorders such as gastroenteritis and inflammatory bowel diseases (IBD), but the species is also found in healthy subjects. The heterogeneous genome of C. concisus increases the likelihood of varying virulence between strains. Flagella motility is a crucial virulence factor for the well‐recognized Campylobacter jejuni; therefore, this study aimed to analyze the motility of C. concisus isolated from saliva, gut biopsies, and feces of patients with IBD, gastroenteritis, and healthy subjects. The motility zones of 63 isolates from 52 patients were measured after microaerobic growth in soft‐agar plates for 72 hours. The motility of C. concisus was significantly lower than that of Campylobacter jejuni and Campylobacter fetus subsp. fetus. The motility of C. concisus varied between isolates (4–22 mm), but there was no statistical significant difference between isolates from IBD patients and healthy subjects (p = 0.14). A tendency of a larger motility zones was observed for IBD gut mucosa isolates, although it did not reach statistical significance (p = 0.13), and no difference was found between oral or fecal isolates between groups. In conclusion, the varying motility of C. concisus could not be related to disease outcome or colonization sites.