Effect of interleukin (IL)-12 and IL-15 on activated natural killer (ANK) and antibody-dependent cellular cytotoxicity (ADCC) in HIV infection

• Published in: Journal of clinical immunology Vol. 18; no. 5; pp. 335 - 345
• Main Authors: LIN, S.-J, ROBERTS, R. L, ANK, B. J, NGUYEN, Q. H, THOMAS, E. K, STIEHM, E. R
• Format: Journal Article
• Language: English
• Published: New York, NY Kluwer/Plenum 01.09.1998; Springer Nature B.V
• Subjects: Adult; AIDS/HIV; Antibody-Dependent Cell Cytotoxicity - immunology; Biological and medical sciences; CD56 Antigen - metabolism; Cells, Cultured; Child; Cytotoxicity, Immunologic - drug effects; Drug Combinations; Female; HIV Envelope Protein gp120 - pharmacology; HIV Infections - immunology; Human viral diseases; Humans; Immunophenotyping; Infectious diseases; Interleukin-12 - pharmacology; Interleukin-15 - pharmacology; Killer Cells, Lymphokine-Activated - drug effects; Killer Cells, Natural - immunology; Medical sciences; Receptors, IgG - metabolism; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Human; Immunopathology; Pathophysiology; Indication; Cytotoxicity; Interleukin 12; AIDS; Interleukin 15; Immune deficiency; Infection; Natural killer cell; Immunomodulator; Mononuclear cell; Mother; Immunological investigation; Viral disease; Antibody-dependent cell cytotoxicity; Child; Lymphocyte
• ISSN: 0271-9142; 1573-2592
• DOI: 10.1023/A:1023290932154

The ability of IL-12 and IL-15 to enhance natural killer (NK) activity and antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells (MNCs) from HIV+ children and their mothers was investigated. MNCs from HIV+ patients were deficient in NK and ADCC activity compared to control MNCs against several target cells. Overnight incubation with IL-15 or IL-12 augmented NK activity of MNCs from both patients and controls, and the combination of IL-12 and IL-15 resulted in the greatest enhancement. ADCC in HIV+ patients against gp120-coated CEM.NKR cells or chicken erythrocytes could also be enhanced by IL-2 or IL-15 in overnight cultures. Culturing MNCs with either IL-2 or IL-15 for 1 week increased the NK activity in patients to levels of controls treated with these cytokines. However, the response to the combination of IL-12 and IL-15 was less than that to IL-15 alone in 1-week cultures. Culturing MNCs with IL-2 and IL-15 for 1 week also increased the percentage of CD16+/CD56+ cells in both patients and controls. Thus, IL-15 can restore the deficient NK activity in patients and may be a candidate for immunomodulative therapy in HIV+ patients.