Analysis of hippocampal gene expression profile of Alzheimer's disease model rats using genome chip bioinformatics

• Published in: Neural regeneration research Vol. 7; no. 5; pp. 332 - 340
• Main Authors: Li, Yinghong, Wu, Zhengzhi, Jin, Yu, Wu, Anmin, Cao, Meiqun, Sun, Kehuan, Jia, Xiuqin, Chen, Manyin
• Format: Journal Article
• Language: English
• Published: India Second Clinical Medical College of Jinan University/Shenzhen Institute of Geriatrics, Shenzhen 518020, Guangdong Provinc%Second Clinical Medical College of Jinan University/Shenzhen Institute of Geriatrics, Shenzhen 518020, Guangdong Province, China 15.02.2012; First Affiliated Hospital of Shenzhen University/Second People's Hospital of Shenzhen City, Shenzhen 518035, Guangdong Province, China%First Affiliated Hospital of Shenzhen University/Second People's Hospital of Shenzhen City, Shenzhen 518035, Guangdong Province, China; Medknow Publications & Media Pvt Ltd
• Subjects: Research and Report: Neurodegenerative Diseases and Neuroregeneration; amyloid beta 1–40; gene expression profile; neural regeneration; hippocampus; genome chip; Alzheimer's disease; amyloid beta 1-40
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.05.002

In this study, an Alzheimer's disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hippocampus of rat models and sham-operated rats were compared by genome expression profiling analysis. Results showed that the expression of 50 genes was significantly up-regulated (fold change ≥ 2), while 21 genes were significantly down-regulated in the hippocampus of Alzheimer's disease model rats (fold change ≤ 0.5) compared with the sham-operation group. The differentially expressed genes are involved in many functions, such as brain nerve system development, neuronal differentiation and functional regulation, cellular growth, differentiation and apoptosis, synaptogenesis and plasticity, inflammatory and immune responses, ion channels/transporters, signal transduction, cell material/energy metabolism. Our findings indicate that several genes were abnormally expressed in the metabolic and signal transduction pathways in the hippocampus of amyloid beta 1-40-induced rat model of Alzheimer's disease, thereby affecting the hippocampal and brain functions.