The effect of vitamin C deficiency and chronic ultraviolet-B exposure on corneal ultrastructure: a preliminary investigation

• Published in: Molecular vision Vol. 17; pp. 3107 - 3115
• Main Authors: Hayes, Sally, Cafaro, Thamara A, Boguslawska, Patrycja J, Kamma-Lorger, Christina S, Boote, Craig, Harris, Jonathan, Young, Robert, Hiller, Jennifer, Terrill, Nicholas, Meek, Keith M, Serra, Horacio M
• Format: Journal Article
• Language: English
• Published: United States Molecular Vision 2011
• Subjects: Animals; Ascorbic Acid Deficiency - pathology; Body Weight; Cornea - pathology; Cornea - radiation effects; Cornea - ultrastructure; Corneal Stroma - pathology; Corneal Stroma - radiation effects; Corneal Stroma - ultrastructure; Guinea Pigs; Male; Microscopy; Scattering, Small Angle; Ultraviolet Rays; X-Ray Diffraction
• ISSN: 1090-0535; 1090-0535

In the visually debilitating condition of climatic droplet keratopathy, corneal transparency is progressively lost. Although the precise cause of the disease and the mechanism by which it progresses are not known, a lifetime exposure to high solar radiation and a vitamin C-deficient diet may be involved in its development. This study examines the effect of dietary ascorbate levels and ultraviolet (UV)-B exposure on corneal stromal structure. Eight guinea pigs were divided into four treatment groups (A, B, C, and D). For 15 weeks, Groups A and C were fed an ascorbate-rich diet (2 mg/100 g bodyweight/day), while Groups B and D received an ascorbate-deficient diet (0.07 mg/100 g bodyweight/day). For the last 12 weeks of the study, Groups C and D also experienced chronic UVB exposure (0.12 J/cm² for 40 min/day). Following euthanasia, the corneas were enucleated and their stromal ultrastructure examined using X-ray scattering and electron microscopy. UVB exposure resulted in an increased corneal thickness (p<0.001), but this was not accompanied by a widespread expansion of the collagen fibrillar array, and in the case of ascorbate-deficient animals, stromal thickening was associated with the compaction of collagen fibrils (p<0.01). Neither UVB exposure nor ascorbic acid deficiency caused any change in the average diameter or D-periodicity of the stromal collagen fibrils. UVB-induced changes in the corneal ultrastructure were most pronounced in animals fed an ascorbic acid-deficient diet. This suggests that ascorbic acid may play a vital role in protecting the corneal stroma from the harmful effects of UVB.