The association of glutathione S-transferase GSTT1 and GSTM1 gene polymorphism with pseudoexfoliative glaucoma in a Pakistani population

• Published in: Molecular vision Vol. 16; pp. 2146 - 2152
• Main Authors: Khan, Muhammad Imran, Micheal, Shazia, Akhtar, Farah, Ahmed, Waqar, Ijaz, Bushra, Ahmed, Asifa, Qamar, Raheel
• Format: Journal Article
• Language: English
• Published: United States Molecular Vision 26.10.2010
• Subjects: Adult; Case-Control Studies; Chromosome Segregation - genetics; Exfoliation Syndrome - complications; Exfoliation Syndrome - enzymology; Exfoliation Syndrome - genetics; Female; Genetic Association Studies; Genetic Predisposition to Disease; Glaucoma - complications; Glaucoma - enzymology; Glaucoma - genetics; Glutathione Transferase - genetics; Humans; Male; Pakistan; Polymerase Chain Reaction; Polymorphism, Single Nucleotide - genetics; Sex Characteristics; Pakistan
• ISSN: 1090-0535; 1090-0535

The aim of the present study was to investigate the association of glutathione S-transferase GSTT1 and GSTM1 genotypes with pseudoexfoliative glaucoma (PEXG) in a group of Pakistani patients. Multiplex polymerase chain reaction was used to study the GSTT1 and GSTM1 polymorphisms in 165 PEXG patients and 162 unaffected controls. In the current study we describe a significant gender-specific association of GSTT1 and GSTM1 null genotypes with PEXG. The three null genotype combinations (i.e., T1M0, T0M1, and T0M0) were found at significantly higher frequencies in the PEXG patients as compared to the controls (χ(2)=21.82, p<0.001). This association was specifically related to the female patients (χ(2)=35.63, p<0.001); no such association was seen in the male patients (χ(2)=2.28, p>0.05). The results suggest that there is a significant involvement of the GSTT1 and GSTM1 polymorphisms in female Pakistani patients having PEXG, which suggests a possible gender-specific impairment of detoxification in this group.