Effect of ground black seeds (Nigella sativa L.) on renal tubular cell apoptosis induced by ischemia/reperfusion injury in the rats

The aim of this study was to evaluate the effects of ground black seeds on renal tubular cell apoptosis following ischemia/reperfusion (I/R) injury in rats. Forty male Wistar rats were randomly allocated into 5 equal groups including Sham, I/R model and three I/R+ black seeds (5, 10 and 20%)-treated...

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Published inIranian journal of basic medical sciences Vol. 17; no. 12; pp. 1032 - 1035
Main Authors Mousavi, Ghafour, Mohajeri, Daryoush
Format Journal Article
LanguageEnglish
Published Iran Mashhad University of Medical Sciences 01.12.2014
Subjects
Short Communication
Rat
Nigella sativa
Ischemia/reperfusion
Kidney
Apoptosis
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Summary:The aim of this study was to evaluate the effects of ground black seeds on renal tubular cell apoptosis following ischemia/reperfusion (I/R) injury in rats. Forty male Wistar rats were randomly allocated into 5 equal groups including Sham, I/R model and three I/R+ black seeds (5, 10 and 20%)-treated groups. I/R groups' kidneys were subjected to 60 min of ischemia followed by 24 h of reperfusion. Microscopically, apoptosis of tubular cells was assessed by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) method. The apoptotic cells of renal tubules were increased significantly (P<0.01) in I/R group than those in sham operation group. The TUNEL positive cells in black seeds (10% and 20%) treatment groups decreased significantly (P<0.05). Inhibition of apoptosis may be responsible for the protective effects of black seeds in rats with renal I/R injury.
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ISSN:2008-3866
2008-3874