Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico

• Published in: Epidemiology and infection Vol. 146; no. 16; pp. 2096 - 2101
• Main Authors: Gutiérrez-Ferman, J L, Villarreal-Treviño, L, Ramírez-Aranda, J M, Camacho-Ortiz, A, Ballesteros-Elizondo, M R, Moreno-Juárez, M R, Mendoza-Olazarán, S, de la O Cavazos, M E, Villarreal-Pérez, J Z, Gómez-Govea, M A, Garza-González, E
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 01.12.2018
• Subjects: Adolescent; Adult; Aged; Antigens; Bacteriological Techniques; Bordetella pertussis; Bordetella pertussis - classification; Bordetella pertussis - genetics; Bordetella pertussis - isolation & purification; Child; Child, Preschool; Deoxyribonucleic acid; DNA; Electrophoresis; Electrophoresis, Gel, Pulsed-Field; Epidemiology; Female; Gel electrophoresis; Gene expression; Genotype; Genotypes; Humans; Immunization; Immunology; Infant; Infant, Newborn; Infections; Infectious diseases; Male; Mexico - epidemiology; Middle Aged; Molecular Epidemiology; Molecular Typing; Original Paper; Outbreaks; Pediatrics; Pertussis; Pertussis Toxin - genetics; Polymerase chain reaction; Population; Pulsed-field gel electrophoresis; Real-Time Polymerase Chain Reaction; Sequence Analysis, DNA; Vaccines; Whooping cough; Whooping Cough - epidemiology; Whooping Cough - microbiology; Young Adult; Mexico; Mexico; pulsed-field gel electrophoresis; Bordetella pertussis; genotyping; ptxP3
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268818002303

We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.