Pseudomonas aeruginosa keratitis: passive immunotherapy with antibodies raised against divalent flagellin

• Published in: Iranian journal of basic medical sciences Vol. 22; no. 1; pp. 58 - 64
• Main Authors: Mahin Samadi, Pariya, Gerami, Parmida, Elmi, Ali, Khanaki, Korosh, Faezi, Sobhan
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.01.2019
• Subjects: Antibiotics; Bacteria; Cornea; Immunoglobulins; Immunotherapy; Laboratories; Molecular weight; Original; Pathogenesis; Pathogens; Research centers; Urinary tract infections; Urogenital system; Divalent antibody; Flagellin; Pseudomonas aeruginosa; Mice; Keratitis
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/ijbms.2018.31499.7643

infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of the infections seem to be still vital. In this report, we aimed to investigate the therapeutic effects of topical administration of the antibodies against type a and b-flagellin (FLA and FLB) in keratitis model of infection in mice. Scratched corneas of mice were treated with approximately 10 CFUs/eye of PAK and/or PAO1 strains of . Specific IgG to FLA, FLB or divalent flagellin were topically applied to the infected corneas for 20 min, 24, and 36 hr post-infection. The bacterial burden and myeloperoxidase activity (as a marker for polymorphonuclears (PMNs) infiltration) were determined in the corneas. The biological activity of the anti-FLA and FLB IgG was evaluated by opsonophagocytosis test. Compared to other treated corneas, divalent anti-flagellin IgG treatment showed a significant decrease in the bacterial CFUs and myeloperoxidase activity in the infected corneas ( <0.05). Results of opsonophagocytosis revealed that the specific antibodies raised against FLA and FLB had more potent opsonic killing activity on their homologous strains as compared with control group ( <0.05). It appears that in keratitis, topical administration of the combined antibodies likely via decreasing the bacterial load, and PMNs infiltration as well as increasing opsonophagocytosis could lead to dramatic improvement of the infected corneas.