Interleukin 1 in Crohn's disease

A number of the activities currently ascribed to the mediator interleukin 1 (IL-1) are relevant to chronic inflammatory bowel disease. Using the mouse thymocyte stimulation assay, lymphocyte-activating factor (LAF) activity was measured in plasma samples and supernatants from cultures of peripheral...

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Published inClinical and experimental immunology Vol. 67; no. 3; pp. 594 - 605
Main Authors SATSANGI, J, WOLSTENCROFT, R. A, CASON, J, AINLEY, C. C, DUMONDE, D. C, THOMPSON, R. P. H
Format Journal Article
LanguageEnglish
Published Oxford Blackwell 01.03.1987
Subjects
Adult
Animals
Biological and medical sciences
Biological Assay
Colitis, Ulcerative - immunology
Crohn Disease - immunology
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Interleukin-1 - analysis
Interleukin-1 - immunology
Lipopolysaccharides - pharmacology
Lymphocyte Activation
Male
Medical sciences
Mice
Mice, Inbred C3H
Middle Aged
Other diseases. Semiology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
T-Lymphocytes - immunology
Crohn disease
Immunological investigation
Interleukin 1
Blood plasma
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Summary:A number of the activities currently ascribed to the mediator interleukin 1 (IL-1) are relevant to chronic inflammatory bowel disease. Using the mouse thymocyte stimulation assay, lymphocyte-activating factor (LAF) activity was measured in plasma samples and supernatants from cultures of peripheral blood mononuclear cells from 16 patients with Crohn's disease, six with ulcerative colitis, and 10 healthy subjects. Results were compared with disease activity, drug therapy, granulocyte count, and plasma levels of zinc and C-reactive protein (CRP). Very low levels of LAF were detected in a few plasma samples from each of the subject groups. Mononuclear cells from healthy subjects produced LAF only when cultured with lipopolysaccharide, but stimulated cells from patients produced greater amounts. Moreover, cells from six patients with Crohn's disease, not receiving steroids, produced LAF spontaneously. Crohn's disease patients also had low plasma zinc but elevated levels of CRP and granulocytes. This enhanced production of LAF in vitro may reflect a primary cellular defect in Crohn's disease, or a secondary consequence of monocyte activation.
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ISSN:0009-9104
1365-2249