Localization of DNA sequences in region Xp21 of the human X chromosome: search for molecular markers close to the Duchenne muscular dystrophy locus

• Published in: American journal of human genetics Vol. 37; no. 2; pp. 235 - 249
• Main Authors: DE MARTINVILLE, B, KUNKEL, L. M, FRANCKE, U, BRUNS, G, MORLE, F, KOENIG, M, MANDEL, J. L, HORWICH, A, LATT, S. A, GUSELLA, J. F, HOUSMAN, D
• Format: Journal Article
• Language: English
• Published: Chicago, IL University of Chicago Press 01.03.1985
• Subjects: Animals; Base Sequence; Biological and medical sciences; Chromosome Banding; Chromosome Mapping; Cricetinae; Cricetulus; Diseases of striated muscles. Neuromuscular diseases; DNA - genetics; DNA Restriction Enzymes; Female; Genetic Linkage; Genetic Markers; Humans; Hybrid Cells; Male; Medical sciences; Mice; Muscular Dystrophies - genetics; Neurology; Nucleic Acid Hybridization; Original; Rats; X Chromosome; Human; Pediatrics; Nervous system diseases; DNA; Duchenne muscular dystrophy; Linkage group; Restriction fragment; X-Chromosome; Molecular cloning; Physical map
• ISSN: 0002-9297; 1537-6605

Panels of somatic cell hybrid lines carrying various structural rearrangements of the human X chromosome short arm were analyzed with 21 X-chromosome-specific cloned DNA fragments. We mapped these molecular markers to five different regions of the short arm of the X chromosome. The results were confirmed by gene-dosage studies of human lymphoblasts with structurally abnormal X chromosomes. The ornithine transcarbamylase gene and four anonymous DNA sequences map within band Xp21, flanking the presumed locus for Duchenne muscular dystrophy.