Enhanced antibacterial properties of biocompatible titanium via electrochemically deposited Ag/TiO2 nanotubes and chitosan-gelatin-Ag-ZnO complex coating

• Published in: RSC advances Vol. 9; no. 8; pp. 4521 - 4529
• Main Authors: Yin, Linling, Fu, Zhenxuan, Li, Yuan, Liu, Bin, Lin, Zongjian, Lu, Jiayu, Chen, Xu, Han, Xiaopeng, Deng, Yida, Hu, Wenbin, Zou, Derong, Zhong, Cheng
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 05.02.2019; The Royal Society of Chemistry
• Subjects: Bacteria; Biocompatibility; Chemistry; Chitosan; Coated electrodes; Gelatin; Nanoparticles; Nanotubes; Silver; Surgical implants; Titanium; Titanium dioxide; Zinc oxide; Zinc oxides
• ISSN: 2046-2069
• DOI: 10.1039/c8ra07682k

A novel double-layered antibacterial coating was fabricated on pure titanium (Ti) via a simple three-step electrodeposition process. Scanning electronic microscopy (SEM) images show that the coating was constructed with the inner layer of TiO 2 nanotubes doped with silver nanoparticles (TNTs/Ag) and the outer layer of chitosan-gelatin mixture with zinc oxide and silver nanoparticles (CS-Gel-Ag-ZnO). In comparison, we also investigated the composition, structure and antibacterial properties of pure Ti coated with TNTs, TNTs/Ag or TNTs/Ag + CS-Gel-Ag-ZnO, respectively. The TNTs was about 100 nm wide and 240 nm to 370 nm tall, and most Ag nanoparticles (Ag NPs) with diameter smaller than 20 nm were successfully deposited inside the tubes. The CS-Gel-Ag-ZnO layer was continuous and uniform. Antibacterial activity against planktonic and adherent bacteria were both investigated. Agar diffusion test against Staphylococcus aureus ( S. aureus ) shows improved antibacterial capacity of the TNTs/Ag + CS-Gel-Ag-ZnO coating, with a clear zone of inhibition (ZOI) up to 14.5 mm wide. Dead adherent bacteria were found on the surface by SEM. The antibacterial rate against planktonic S. aureus was as high as 99.2% over the 24 h incubation period. A novel complex antibacterial coating fabricated via a simple three-step electrodeposition process shows high antibacterial rate of 99.2%.