In vivo transplantation of genetically modified mouse embryonic epidermis

Situations in which epidermal mutant mice display early lethality after birth are rather frequent. This condition precludes any kind of analysis of adult or even newborn mice tissues. We propose the in vivo embryonic skin transplantation as an alternative to solve this problem. This method allows th...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 585; p. 361
Main Authors Martínez-Cruz, Ana Belén, Costa, Clotilde, Saiz, Cristina, Paramio, Jesús M, Santos, Mirentxu
Format Journal Article
LanguageEnglish
Published United States 01.01.2010
Subjects
Animals
Cell Differentiation
Embryo, Mammalian - metabolism
Epidermis - embryology
Epidermis - transplantation
Female
Genotype
Mice
Phenotype
Skin Transplantation - methods
Tissue Engineering
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Summary:Situations in which epidermal mutant mice display early lethality after birth are rather frequent. This condition precludes any kind of analysis of adult or even newborn mice tissues. We propose the in vivo embryonic skin transplantation as an alternative to solve this problem. This method allows the generation of a stable epidermal tissue that mimics the specific mutant adult epidermis. It also reproduces the phenotypic consequences and susceptibility to tumorigenesis, enabling multiple studies. Moreover, different recipient mice can help in determining problems such as different inflammation processes or the contribution of dermal cells to the different phenotypes.
ISSN:1940-6029
DOI:10.1007/978-1-60761-380-0_25