Inhibition of SV40 gene expression by microinjected small antisense RNA and DNA molecules

We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecul...

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Bibliographic Details
Published inNucleic acids research Vol. 19; no. 1; pp. 53 - 59
Main Authors GRAESSMANN, M, MICHAELS, G, BERG, B, GRAESSMANN, A
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 11.01.1991
Subjects
Animals
Antigens, Polyomavirus Transforming - biosynthesis
Base Sequence
Biological and medical sciences
Blotting, Northern
Cell Line
Computer Simulation
DNA, Antisense - pharmacology
DNA, Viral - drug effects
DNA, Viral - pharmacology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression - drug effects
Genes, Viral
Kinetics
Microinjections
Molecular and cellular biology
Molecular Biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
Rats
RNA, Antisense - pharmacology
RNA, Viral - drug effects
RNA, Viral - pharmacology
Simian virus 40
Simian virus 40 - drug effects
Simian virus 40 - genetics
Polyomavirus
Monkey
Antisense RNA
Papovaviridae
Gene expression
Secondary structure
Virus
Vertebrata
Mammalia
Cell line
Microinjection
Messenger RNA
Gene
Primates
SV40 virus
Antisens DNA
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Summary:We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.
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To whom correspondence should be addressed
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/19.1.53