Laparoscopic embryo transfer in pigs - comparison of different variants and efficiencies of the method

The aim of the study was to develop a method of laparoscopic embryo transfer in pigs and to compare different variants of this method. Two catheter diameters (1.6 mm and 1.0 mm), the method and site of embryo deposition (oviduct or uterus), the embryo development stage (2 - 4 cell or blastocyst), th...

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Bibliographic Details
Published inPolish journal of veterinary sciences Vol. 26; no. 2; pp. 295 - 306
Main Authors Wieczorek, J, Stodolak-Zych, E, Okoń, K, Koseniuk, J, Bryła, M, Jura, J, Poniedziałek-Kempny, K, Rajska, I, Sobol, K, Kotula Balak, M, Chmurska-Gasowska, M
Format Journal Article
LanguageEnglish
Published Germany Polish Academy of Sciences, Committee of Veterinary Sciences 01.06.2023
Subjects
Catheters
Cryopreservation
Developmental stages
Efficiency
Embryo transfer
Embryos
Fallopian tube
Fallopian tubes
Hogs
Laparoscopy
Methods
Oviduct
Performance evaluation
Uterus
laparoscopy
embryo
transfer
pig
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Summary:The aim of the study was to develop a method of laparoscopic embryo transfer in pigs and to compare different variants of this method. Two catheter diameters (1.6 mm and 1.0 mm), the method and site of embryo deposition (oviduct or uterus), the embryo development stage (2 - 4 cell or blastocyst), the method for oviduct or uterus stabilization, the potential for cryopreserved embryo transfer, the developmental potential of the embryos after transfer to the oviduct, patomorphology of the oviduct after transfer and possible clinical complications were taken into consideration. Two studies compared two variants of transfer to the uterus, and five variants of transfer to the fallopian tube. The transfer of embryos by the infundibulum may be of limited use due to handling problems and very low efficiency (pregnancy was not achieved). Very low efficiency was shown after transfer of vitrified embryos. Transfer to the fallopian tube by puncture of the fallopian tube, regardless of the developmental stage of the embryo, is the recommended method of embryo transfer. The histopathological examination of the fallopian tube revealed possible changes within the puncture site. The numerous clinical complications observed did not affect the effectiveness of the method.
ISSN:1505-1773
2300-2557
DOI:10.24425/pjvs.2023.145036