Repeated measurements of P retention in ponies fed rations with various Ca:P ratios

• Published in: Journal of animal science Vol. 92; no. 11; pp. 4981 - 4990
• Main Authors: van Doorn, D A, Schaafstra, F J W C, Wouterse, H, Everts, H, Estepa, J C, Aguilera-Tejero, E, Beynen, A C
• Format: Journal Article
• Language: English
• Published: United States 01.11.2014
• Subjects: Animals; Body Weight - drug effects; Bone and Bones - drug effects; Bone and Bones - metabolism; Calcium - metabolism; Calcium, Dietary - pharmacology; Diet - veterinary; Horses - metabolism; Magnesium - metabolism; Male; Parathyroid Hormone - metabolism; Phosphorus - metabolism; Phosphorus, Dietary - pharmacology; Time Factors; magnesium; bone; calcium; balance; retention; phosphorus
• ISSN: 1525-3163
• DOI: 10.2527/jas.2014-7632

This study addresses the question of whether feeding rations rich in P for a period of up to 42 d induces a positive P balance in adult ponies. Biochemical bone markers and parathyroid hormone (PTH; intact as well as whole PTH) were measured to obtain clues as to the effect of P loading on bone metabolism. The experiment had a Latin square design. Each feeding period lasted 42 d, and there were 2 balance trials (ECP1 and ECP2) within each feeding period. Each balance trial lasted 10 d (ECP1: d 11 to 21; ECP2: d 33 to 42). Six ponies aged 2.5 to 7 yr were fed a control diet that provided P and Ca according to the requirement (Control diet: 54 mg Ca·kg BW(-1) · d(-1); 36 mg P · kg BW(-1) · d(-1)), a diet high in Ca and P (HCaHP diet: 146 mg Ca · kg BW(-1) · d(-1); 121 mg P · kg BW(-1) · d(-1)), and a diet with a high P level only and Ca fed to the requirement (HP diet: 54 mg Ca · kg BW(-1) · d(-1); 122 mg P · kg BW(-1) · d(-1)). When fed the Control diet, the ponies showed a zero P and Ca balance over the 42-d period. The HCaHP diet resulted in both P and Ca retention (about 2 g Ca and P/d; P < 0.05). Phosphorus retention (about 2 g P/d) alone was observed when ponies were fed the HP diet, but P retention was only different (P < 0.05) from the Control diet in ECP1. The excretion of P in urine was reduced by greater Ca intake (P < 0.05), and Mg absorption was reduced by high P intake (P < 0.05). Plasma P concentration was raised by high P intake. Plasma Ca levels were not affected by dietary treatment. The greater (P < 0.05) P retentions observed for the HCaHP diet during ECP1 and ECP2 and HP diet during ECP1 could not be explained by processes that could have been indicated by the bone markers or PTH values. It was concluded that dietary-P-induced retention of P in ponies does not seem to be associated with altered bone metabolism in this study.