Hyperbaric oxygen in skeletal muscle of rats submitted to total acute left hindlimb ischemia: A research report

• Published in: Undersea & hyperbaric medicine Vol. 42; no. 1; p. 41
• Main Authors: da Silva, Luis Gustavo Campos, Dalio, Marcelo Bellini, Joviliano, Edwaldo Edner, Feres, Omar, Piccinato, Carlos Eli
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Acute Disease; Animals; Aspartate Aminotransferases - analysis; Creatine Kinase - analysis; Glycogen - analysis; Hindlimb - blood supply; Hyperbaric Oxygenation; Ischemia - metabolism; Ischemia - therapy; L-Lactate Dehydrogenase - analysis; Male; Malondialdehyde - analysis; Muscle, Skeletal - blood supply; Muscle, Skeletal - metabolism; Random Allocation; Rats; Rats, Wistar; Research Report; Serum Albumin - analysis; Time Factors
• ISSN: 1066-2936

Determine the effect of hyperbaric oxygen treatment in skeletal muscle of rats submitted to total acute left hindlimb ischemia. An experimental study was designed using 48 Wistar rats divided into four groups (n = 12): Control; Ischemia (I)--total hindlimb ischemia for 270 minutes; Hyperbaric oxygen treatment during ischemia (HBO2)--total hindlimb ischemia for 270 minutes and hyperbaric oxygen during the first 90 minutes; Pre-treatment with hyperbaric oxygen (PHBO2)--90 minutes of hyperbaric oxygen treatment before total hindlimb ischemia for 270 minutes. Skeletal muscle injury was evaluated by measuring levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total creatine phosphokinase (CPK); muscular malondialdehyde (MDA), muscular glycogen, and serum ischemia-modified albumin (IMA). AST was significantly higher in I, HBO2 and PHBO2 compared with control (P = .001). There was no difference in LDH. CPK was significantly higher in I, HBO2 and PHBO2, compared with control (p = .014). MDA was significantly higher in PHBO2, compared with other groups (p = .042). Glycogen was significantly decreased in I, HBO2 and PHBO2, compared with control (p < .001). Hyperbaric oxygen treatment in acute total hindlimb ischemia exerted no protective effect on muscle injury, regardless of time of application. When applied prior to installation of total ischemia, hyperbaric oxygen treatment aggravated muscle injury.